Activation of phosphoenolpyruvate‐dependent protein kinase by cytidine 5′‐triphosphate in rat skeletal muscle

FEBS Letters

Waygood, E.Bruce¹  Mattoo, Roshan L.¹  Khandelwal, Ramji L.¹ 

[1] Department of Biochemistry, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0W0, Canada

关键词: Protein kinase;    Phosphoenol pyruvate;    Skeletal muscle;    Phosphorylation;    Cytidine triphosphate;    Phosphoprotein;   

DOI  :  10.1016/0014-5793(84)80026-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of nucleotides on phosphoenolpyruvate-dependent protein kinase in rat skeletal muscle were examined. Various ribonucleotide triphosphates were tested and the protein kinase reaction was maximally activated by cytidine 5′-triphosphate followed by uridine 5′-triphosphate and guanosine 5′-triphosphate. No activation by adenosine 5′-triphosphate was observed. Cytidine 5′-diphosphate also activated the reaction but to a significantly lesser extent. Cytidine 5′-monophosphate and cyclic cytidine 3′,5′-monophosphate showed no effect whereas cytidine was slightly inhibitory.

【 授权许可】

Unknown