FEBS Letters | |
Acrosome membrane associated protein kinase activity in hamster spermatozoa | |
Ahmad, M.Basheer1  Devi, K.Uma1  Shivaji, S.1  | |
[1] Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India | |
关键词: Phosphorylation; Sperm maturation; Membrane protein; Protein kinase; Acrosomal membrane; Hamster spermatozoa; Tyrosine specific protein kinase; | |
DOI : 10.1016/0014-5793(96)01077-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Acrosomal membranes isolated from the caput and cauda epididymal spermatozoa of hamster exhibited protein kinase activity and the endogenous protein substrates that were phosphorylated in the acrosomal membranes of caput and cauda spermatozoa were not all the same. The kinase activity was identified as a cAMP independent type and the use of specific stimulators and inhibitors indicated that the activity was not due to casein kinase, protein kinase A or protein kinase C but due to a tyrosine specific protein kinase that was not inhibited by Genistein. Phosphotyrosine was identified as the predominant phosphorylated residue in the proteins.
【 授权许可】
Unknown
【 预 览 】
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RO201912020303436ZK.pdf | 439KB | download |