The regulation of the proton conductance of brown fat mitochondria

FEBS Letters

Rial, E.¹  Nicholls, D.G.¹ 

[1] Neurochemistry Laboratory, Dept. of Psychiatry, University of Dundee, Dundee DD1 9 SY, Scotland

关键词: Brown fat;    Mitochondria;    Purine nucleotide;    Proton conductance;    Swelling;    Binding;    Tes;    2[2-hydroxy-1;    1-bis[hydroxymethyl]-ethyl)amine]-ethane sulphonate;   

DOI  :  10.1016/0014-5793(83)81026-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding of purine nucleotides to intact brown fat mitochondria is re-examined. In addition to the previously reported high affinity binding site, a low-affinity site is found, which requires several minutes to saturate. Only the high affinity site is functional in regulating the proton and halide permeabilities of the mitochondria. The low affinity site can introduce errors in the use of nucleotide binding to quantitate the M _r 32 000 uncoupling protein unique to these mitochondria.

【 授权许可】

Unknown