FEBS Letters | |
Diamide blocks H+ conductance in mitochondrial H+‐ATPase by oxidizing FB dithiol | |
Huang, Youguo1  Pringle, Michael J.1  Rao Sanadi, D.1  | |
[1] Department of Cell Physiology, Boston Biomedical Research Institute, 20 Staniford Street, Boston, MA 02114 USA | |
关键词: Diamide; Cadmium; Proton conductance; Sulfhydryl group; F0; H+-ATPase; Mitochondria; | |
DOI : 10.1016/0014-5793(85)80048-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Effects of diamide on proton conductance of electron transport particles (ETPH), purified H+-ATPase (F1–F0), F0 of the H+-ATPase from beef heart mitochondria and binding of cadmium (109Cd) to the H+-ATPase have been examined in the present paper. When ETPH and purified H+-ATPase are treated with 1 mM diamide, ATP-dependent generation of membrane potential, monitored by the absorbance change produced by the redistribution of oxonol VI, is consistantly inhibited. Diamide also blocks passive H+ conductance driven by a K+ diffusion potential in the membrane sector, F0 of H+-ATPase. Furthermore, diamide treatment drastically reduces the binding of 109Cd2+ to H+-ATPase, showing competition for the FB dithiol group.
【 授权许可】
Unknown
【 预 览 】
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RO201912020287342ZK.pdf | 421KB | download |