Diamide blocks H+ conductance in mitochondrial H+‐ATPase by oxidizing FB dithiol

FEBS Letters

Huang, Youguo¹  Pringle, Michael J.¹  Rao Sanadi, D.¹ 

[1] Department of Cell Physiology, Boston Biomedical Research Institute, 20 Staniford Street, Boston, MA 02114 USA

关键词: Diamide;    Cadmium;    Proton conductance;    Sulfhydryl group;    F0;    H+-ATPase;    Mitochondria;   

DOI  :  10.1016/0014-5793(85)80048-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Effects of diamide on proton conductance of electron transport particles (ETPH), purified H⁺-ATPase (F₁–F₀), F₀ of the H⁺-ATPase from beef heart mitochondria and binding of cadmium (¹⁰⁹Cd) to the H⁺-ATPase have been examined in the present paper. When ETPH and purified H⁺-ATPase are treated with 1 mM diamide, ATP-dependent generation of membrane potential, monitored by the absorbance change produced by the redistribution of oxonol VI, is consistantly inhibited. Diamide also blocks passive H⁺ conductance driven by a K⁺ diffusion potential in the membrane sector, F₀ of H⁺-ATPase. Furthermore, diamide treatment drastically reduces the binding of ¹⁰⁹Cd²⁺ to H⁺-ATPase, showing competition for the F_B dithiol group.

【 授权许可】

Unknown