Effect of acclimation temperature on the concentration of the mitochondrial ‘uncoupling’ protein measured by radioimmunoassay in mouse brown adipose tissue

FEBS Letters

Stirling, Dorothy M.¹  Ashwell, Margaret¹  Jennings, Graham¹  Richard, Denis¹  Trayhurn, Paul¹ 

[1] Dunn Nutrition Laboratory, Medical Research Council and University of Cambridge, Downham's Lane, Milton Road, Cambridge CB4 1XJ, England

关键词: Brown adipose tissue;    Uncoupling protein;    Mitochondria;    Radioimmunoassay;    GDP binding;    Proton conductance;   

DOI  :  10.1016/0014-5793(83)80740-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of acclimation temperature on the concentration of the mitochondrial ‘uncoupling’ protein (M _r 32 000) from brown adipose tissue of mice has been investigated. The uncoupling protein was measured by a specific radioimmunoassay. Between 33°C (thermoneutrality) and −2°C there was a progressive increase with decreasing environmental temperature in the amount of uncoupling protein. For mice at −2°C the mitochondrial concentration of the protein was 9-times higher than at 33°C, while the total amount of the protein in interscapulaar brown adipose tissue was estimated to be nearly 80-times greater at −2°C compared to 33°C.

【 授权许可】

Unknown