Molecular mechanism of uncoupling in brown adipose tissue mitochondria

FEBS Letters

Kopecký, Jan¹  Guerrieri, Ferruccio²  Drahota, Zdeněk¹  Houštěk, Josef¹  Ježek, Petr¹ 

[1] Institute of Physiology, Czechoslovak Academy of Sciences, Videňská 1083, 14220 Prague, Czechoslovakia;Institute of Biological Chemistry, University of Bari, Policlinico, Piazza G. Cesare, 70124 Bari, Italy

关键词: Brown adipose tissue;    Mitochondria;    Uncoupling protein;    Proton conductance;    Anion conductance;    pH measurement;    BAT;    brown adipose tissue;    CCCP;    carbonylcyanide m-chlorophenylhydrazone;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(84)81396-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Specific permeability properties of the inner membrane of brown adipose tissue mitochondria were analysed with the aid of simultaneous pH measurements outside mitochondria and of mitochondria swelling. It was shown that valinomycin-induced potassium diffusion potential drives a parallel passive uptake of chlorides and extrusion of proton. Electrogenic H⁺-extrusion was independent on anion transport, no competition was found between the two processes and the former process exerted a lower sensitivity to the inhibitory effect of GDP. The existence of two distinct, independent pathways for translocation of protons and halide anions across the membrane is suggested.

【 授权许可】

Unknown