Chemical modification of the brown fat mitochondrial uncoupling protein with tetranitromethane

FEBS Letters

Nicholls, David G.¹  Rial, Eduardo¹ 

[1] Neurosciences Research Group, Department of Psychiatry, Ninewells Medical School, University of Dundee, Dundee DD1 4HN, Scotland

关键词: (Brown fat);    Chemical modification;    Mitochondria;    Purine nucleotide;    Tetranitromethane Uncoupling protein;   

DOI  :  10.1016/0014-5793(86)81178-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tetranitromethane reacts with the uncoupling protein of intact brown fat mitochondria. The chloride permeability in the absence of the inhibitory nucleotide GDP is not affected, but the affinity with which GDP binds is decreased, and the coupling between binding of nucleotide and inhibition of chloride permeation is broken.

【 授权许可】

Unknown