Release of leukotriene C4 from human polymorphonuclear leucocytes as determined by radioimmunoassay

FEBS Letters

Aehringhaus, U.³  König, W.¹  Wölbling, R.H.³  Peskar, B.M.³  Patrono, C.²  Peskar, B.A.³ 

[1] Institut für Medizinische Mikrobiologie und Immunologie, Arbeitsgruppe Infektabwehrmechanismen, Ruhr-Universität Bochum, D-4630 Bochum 1, FRG;Department of Pharmacology, Catholic University, I-00168 Rome, Italy;Institut für Pharmakologie und Toxikologie der Ruhr-Universität Bochum, Im Lottental, D-4630 Bochum 1, FRG

关键词: Leukotriene C4;    Radioimmunoassay;    Leucocyte;    Bioassay;    Slow-reacting substance;    Lipoxygenase;   

DOI  :  10.1016/0014-5793(82)80715-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rabbits were immunized with a conjugate of leukotriene (LT) C₄ and bovine serum albumin prepared by coupling the single free amino group of the hapten to the protein using gluteraldehyde. Binding of [³H]LTC₄ to the antibodies obtained is inhibited by 50% with 1.5 ng LTC₄. The relative cross-reaction of LTD₄ is 16% and of LTC₄-methyl ester 3.6%. The validity of the radioimmunoassay was demonstrated by comparison with bioassay using the isolated guinea pig ileum. Using the radioimmunoassay it could be shown that endogenous LTC₄ is released in a dose-dependent manner by human polymorphonuclear leucocytes stimulated with the divalent cation ionophore A23187.

【 授权许可】

Unknown