A sensitive and specific radioimmunoassay for leukotriene C4

FEBS Letters

Lindgren, Jan Åke²  Hammerström, Sven²  Goetzl, Edward J.¹ 

[1] Howard Hughes Medical Institute Laboratories and Division of Allergy and Immunology, University of California Medical Center, San Francisco, CA 94143, USA;Department of Physiological Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden

关键词: Radioimmunoassay;    Leukotriene C4;    6-Sulfidopeptide specificity;    Leukocyte suspension;    Ionophore A23187-stimulation;    Leukotriene C4-formation;   

DOI  :  10.1016/0014-5793(83)80487-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A sensitive and specific radioimmunoassay suitable for direct measurement of leukotrine C₄ was developed. Acetylated leukotriene C₄ was coupled to polyamino bovine serum albumin using 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide hydrochloride as coupling agent. The conjugate in complete or incomplete Freund's adjutant was injected into New Zealand white rabbits. At a final antiplasma dilution of 1 : 1050 the lowest detection limit of leukotriene C₄ was 0.046 pmol. The antiplasma cross-reacted <1% with leukotrienes D₄, E₄ and F₄, while high relative cross-reaction (86–100%) was obtained with leukotrienes C₃, C₅ and 11-trans leukotriene C₄. In experiments where known amounts of leukotriene C₄ were added to leukocytes suspensions, 67–100% of the added amount was recovered by the method. The radioimmunoassay was used to study leukotriene C₄ formation after stimulation of leukocyte suspensions with the ionophore A23187.

【 授权许可】

Unknown