Identification of organic anion transporting polypeptide 4 (Oatp4) as a major full‐length isoform of the liver‐specific transporter‐1 (rlst‐1) in rat liver

FEBS Letters

Winterhalter, Kaspar E.¹  Hagenbuch, Niels²  Meier, Peter J.²  Ha, Riem³  Hagenbuch, Bruno²  Stieger, Bruno²  Cattori, Valentino² 

[1] Institute of Biochemistry, Department of Biology, Swiss Federal Institute of Technology (ETH), CH-8092 Zurich, Switzerland;Division of Clinical Pharmacology and Toxicology, Department of Medicine, University Hospital Zurich, CH-8091 Zurich, Switzerland;Cardiovascular Therapy Research Group, Department of Medicine, University Hospital, CH-8091 Zurich, Switzerland

关键词: Liver transport;    Bile salt;    Sulfobromophthalein;    Steroid conjugate;    Thyroid hormone;    Leukotriene C4;    BSP;    sulfobromophthalein;    LST-1(OATP-C;    OATP2);    human liver specific transporter-1;    Oatp;    rat organic anion transporting polypeptide;    OATP;    human organic anion transporting polypeptide;    OAT-K;    rat kidney organic anion transporting polypeptide;    PGT;    prostaglandin transporter;    rlst-1;    rat liver specific transporter-1;   

DOI  :  10.1016/S0014-5793(00)01596-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel organic anion transporting polypeptide (Oatp)4¹ was isolated from rat liver that is 35 amino acids longer than the reported rat liver specific organic anion transporter (rlst)-1 and exhibits a 64% amino acid sequence identity with the human OATP-C (LST-1/OATP2; gene symbol SLC21A6). When expressed in Xenopus laevis oocytes, Oatp4 (Slc21a10) mediated polyspecific uptake of a variety of organic anions including taurocholate (K _m∼27 μM), bromosulfophthalein (K _m∼1.1 μM) and steroid conjugates. Based on nuclease protection analysis Oatp4 appears to be the predominant transcript in rat liver indicating that rlst-1 plays a minor role in overall hepatic organic anion uptake.

【 授权许可】

Unknown