Interactions of bile salt micelles and colipase studied through intermolecular nOes

FEBS Letters

Guerlesquin, Françoise¹  Chapus, Catherine¹  Sebban-Kreuzer, Corinne¹  Bornet, Olivier¹  Dominguez, Cyril¹  Kerfelec, Brigitte¹ 

[1] Unité de Bioénergétique et Ingénierie des Protéines, IBSM-CNRS, 31 chemin Joseph Aiguier, 13204 Marseille Cedex 20, France

关键词: Colipase;    Bile salt;    Micelle;    Nuclear magnetic resonance;    Intermolecular nuclear Overhauser effect;    CMC;    critical micellar concentration;    NaTDC;    sodium taurodeoxycholate;    NMR;    nuclear magnetic resonance;    nOe;    nuclear Overhauser effect;    NOESY;    nuclear Overhauser effect spectroscopy;    TOCSY;    total correlation spectroscopy;   

DOI  :  10.1016/S0014-5793(00)02034-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Colipase is a small protein (10 kDa), which acts as a protein cofactor for the pancreatic lipase. Various models of the activated ternary complex (lipase–colipase–bile salt micelles) have been proposed using detergent micelles, but no structural information has been established with bile salt micelles. We have investigated the organization of sodium taurodeoxycholate (NaTDC) micelles and their interactions with pig and horse colipases by homonuclear nuclear magnetic resonance (NMR) spectroscopy. The NMR data supply evidence that the folding of horse colipase is similar to that already described for pig colipase. Intermolecular nuclear Overhauser effects have shown that two conserved aromatic residues interact with NaTDC micelles.

【 授权许可】

Unknown