Specific sequence‐directed anti‐bilitranslocase antibodies as a tool to detect potentially bilirubin‐binding proteins in different tissues of the rat

FEBS Letters

Luigi Sottocasa, Gian¹  Micali, Fulvio¹  Passamonti, Sabina¹  Battiston, Lucia¹  Macagno, Annalisa¹ 

[1] Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via Giorgieri 1, I-34100 Trieste, Italy

关键词: Bilitranslocase;    Bilirubin;    Organic anion transport;    Liver transport;    Tissue distribution of bilitranslocase;    CNS;    central nervous system;    MAP;    multiple antigen peptide;    SDS;    sodium dodecyl sulfate;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    EDTA;    ethylenediaminetetraacetic acid;    TBS;    Tris-buffered saline;    BSA;    bovine serum albumin;   

DOI  :  10.1016/S0014-5793(99)00736-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hypothesis that the uneven distribution of bilirubin in the organism, which occurs in hyperbilirubinemia, could reflect an uneven distribution of bilirubin-binding proteins was tested by searching for peptides containing the bilirubin-binding motif identified in bilitranslocase (Battiston et al., 1998). In the rat, positive proteins bands were found to be present only in the liver, gastric mucosa and central nervous system. The electrophoretic mobilities of the positive compounds in the liver and stomach were identical to that of purified bilitranslocase (38 kDa). In the brain, on the contrary, two peptides were found with molecular masses of 79 and 34 kDa, respectively. Their distribution pattern in the central nervous system was different for each of them.

【 授权许可】

Unknown