Molecular cloning and characterization of two novel transport proteins from rat kidney

FEBS Letters

Engelhardt, Martin¹  Gründemann, Dirk¹  Schömig, Edgar¹  Örding, Nicola¹  Spitzenberger, Folker¹  Martel, Fátima¹ 

[1] Department of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg, Germany

关键词: Organic cation transport;    Organic anion transport;    Amphiphilic solute facilitator family;    Phylogeny;    Evolution;    RT;    reverse transcriptase;    PCR;    polymerase chain reaction;    TM;    transmembrane segment;    kb;    kilobase(s);    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;   

DOI  :  10.1016/S0014-5793(98)00203-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The recent cloning of renal transport systems for organic anions and cations (OAT1, OCT1, and OCT2) opened the possibility to search, via polymerase chain reaction (PCR) homology screening, for novel transport proteins. Two integral membrane proteins, UST1 and UST2, were cloned from rat kidney. RT-PCR revealed that UST1 is confined to the kidney whereas UST2 mRNA was detected in all tested tissues. Sequence analyses suggest that UST1 and UST2, together with four related transporters, comprise, within the major facilitator superfamily, a so far unrecognized transporter family, termed amphiphilic solute facilitator (ASF) family. Characteristic signatures for the ASF family were identified.

【 授权许可】

Unknown