Formation of leukotriene C4 by human leukocytes exposed to monosodium urate crystals

FEBS Letters

Samuelsson, Bengt²  Lindgren, Jan Åke²  Weissmann, Gerald¹  Lundberg, Ulf²  Serhan, Charles N.² 

[1] Division of Rheumatology, Department of Medicine, New York University School of Medicine, 550 First Avenue, NY 10016, USA;Department of Physiological Chemistry, Karolinska Institutet, S-10401 Stockholm, Sweden

关键词: Leukotriene C4;    Monosodium urate crystal;    Radioimmunoassay;    Human leukocyte;   

DOI  :  10.1016/0014-5793(84)80842-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monosodium urate (MSU) crystals stimulate the metabolism of arachidonic acid in mixed populations of human leukocytes. Leukocytes exposed to MSU crystals released leukotriene C₄. Leukotriene C₄ (LTC₄) was characterized and detected by high-performance liquid chromatography (HPLC), UV absorption, bioassay with guinea pig ileum, and radioimmunoassay. Results indicate that MSU crystals stimulate the transformation of arachidonic acid and the formation of leukotriene C₄ in human leukocytes; an effect inhibited by colchicine. Moreover, they suggest that LTC₄ may serve as a mediator of inflammation in crystal-associated diseases.

【 授权许可】

Unknown