Effects of Stephania hainanensis alkaloids on MSU-induced acute gouty arthritis in mice

BMC Complementary Medicine and Therapies

Yi-qian Xu¹  Hao Jia¹  Dao-rui Yu¹  Hao-lin Wu¹  Hao-fei Fan¹  Qi-bing Liu¹  Xing-yue Fang²  Li-chong Gong³  Xiao-liang Tang⁴ 

[1] Department of Pharmacology, School of Basic and Life Science, Hainan Medical University, 571199, Haikou, China;HMC Cancer Institute, The First Affiliated Hospital of Hainan Medical University, Haikou, China;Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School, 02129, Boston, MA, USA;School of Traditional Chinese Medicine, Hainan Medical University, 571199, Haikou, China;

关键词: Gouty arthritis;    Monosodium urate crystal;    Stephania hainanensis;    Inflammasome;   

DOI  :  10.1186/s12906-021-03364-5

来源: Springer

【 摘 要 】

BackgroundGout is initiated by the precipitation of monosodium urate (MSU) crystals within the joints and soft tissues, and it can eventually cause acute or chronic arthritis. MSU crystals trigger, amplify, and maintain a strong inflammatory response through promoting proinflammatory activity. In this study, the therapeutic effects of Stephania hainanensis (S. hainanensis) total alkaloid (SHA) were tested and evaluated on MSU-induced acute gouty arthritis in a mouse model.MethodsAfter oral administration of SHA (10 or 20 mg/kg) or the antigout medicine colchicine (0.5 mg/kg) once daily for 3 consecutive days, MSU crystals suspended in saline (2.5 mg/50 μl) were intradermally injected into the right paw of the mice. Then, SHA and colchicine were administered for another 2 days. During this period, swelling of the ankle and clinical scores were measured at 12, 24, and 48 h postinjection. After the mice were euthanized, inflammatory cytokine expression and paw tissue inflammation-related gene and protein expression, and a histopathological analysis was performed.ResultsSHA had obvious therapeutic effects on MSU-induced acute gouty arthritis in mice. SHA alleviated ankle swelling and inhibited the production of cytokines, such as IL-1β and TNF-α. In addition, NLRP3, Caspase-1 and IL-1β, which are activated by MSU were also suppressed by SHA. The histological evaluation showed that SHA relieved the infiltration of inflammation around the ankle.ConclusionsThese results suggest that SHA is capable of anti-inflammatory activities and may be useful for treating gouty arthritis.

【 授权许可】

CC BY