Isolation of glucagon‐37 (bioactive enteroglucagon/oxyntomodulin) from porcine jejuno‐ileum

FEBS Letters

Bataille, D.¹  Rosselin, G.¹  Gespach, C.¹  Mutt, V.³  Jörnvall, H.²  Tatemoto, K.³ 

[1] INSERM U.55, Hôpital Saint-Antoine, 75571 Paris Cedex 12, France;Department of Biochemistry I, Karolinska Institute, S 10401 Stockholm, Sweden;Department of Biochemistry II, Karolinska Institute, S 10401 Stockholm, Sweden

关键词: Glucagon-37;    Oxyntomodulin;    Bioactive enteroglucagon;    Amino acid sequence;    Immunology;    Biological activity;   

DOI  :  10.1016/0014-5793(82)80709-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A peptide isolated from porcine gut according to its glucagon-like activity in liver (bioactive enteroglucagon) has been characterized immunologically, biologically and chemically: its potency relative to pancreatic glucagon in interacting with an antiglucagon antibody, hepatic glucagon-binding sites and hepatic adenylate cyclase was ∼100%, 20% and 10%, respectively. In contrast, it is ∼20-times more potent than glucagon in oxyntic glands, justifying the term ‘oxyntomodulin’. Chemically, it consists in the 29 amino acid-peptide glucagon elongated at its C-terminal end by the octapeptide Lys—Arg—Asn—Lys—Asn—Asn—Ile &—Ala; accordingly, it is called ‘glucagon-37’

【 授权许可】

Unknown