Comparative study of three Marantodes pumilum varieties by microscopy, spectroscopy and chromatography

Revista Brasileira de Farmacognosia

Jalil, Juriyati¹  Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia¹  Hamsani, Nur Ain M.¹  Sabandar, Carla W.¹  Talip, Noraini¹  Universiti Kebangsaan Malaysia, Malaysia¹  Aladdin, Nor-Ashila¹  Jamal, Jamia Azdina¹  Rahman, Mohd Ruzi A.¹  Husain, Khairana¹  Muhammad, Kartiniwati¹ 

关键词:  Anatomy;    ATR-FTIR;    HPLC;    HPTLC;    Marantodes pumilum;    Microscopy;   

DOI  :  10.1016/j.bjp.2015.10.002

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Marantodes pumilum (Blume) Kuntze (synonym: Labisia pumila (Blume) Fern.-Vill), Primulaceae, is well known for its traditional use as a post-partum medication among women in Malaysia. Three varieties of M. pumilum, var. alata Scheff., var. pumila and var. lanceolata (Scheff.) Mez. are commonly used. Nowadays, M. pumilum powder or extracts are commercially available as herbal supplements and beverages. Authentication of the variety is an important component of product quality control. Thus, the present work was aimed to compare the three varieties using microscopic, spectroscopic and chromatographic techniques. Microscopic anatomical examination and powder microscopy were performed on fresh and dried plant materials, respectively. Fingerprint profiles of the varieties were obtained using attenuated total reflectance-Fourier transform infrared spectrophotometer, high performance thin layer chromatography and high performance liquid chromatography. The microscopic examination showed presence of anisocytic stomata, scale and capitate glandular trichome in all varieties. The type of stomata and trichomes, outline structure of stem and leaf margin, petiole and midrib, organization of vascular system, areolar venation, pattern of anticlinal walls, the distribution of secretory canals and cell inclusion as well as the measurement of selected structures could be used to distinguish and identify each variety of M. pumilum. In addition, spectroscopic and chromatographic fingerprint analyses of the three varieties exhibited distinguishable profiles based on the intensity of certain peaks or bands. The findings from this study will provide systematic identification for these varieties.

【 授权许可】

Unknown