The retarded rate of acid‐catalyzed solvolysis of glycoside bonds between reducing‐end glucose residue and ceramide in glycosphingolipids compared with that of glycoside bonds between hexopyranosides

FEBS Letters

Shimamura, Michio¹  Oku, Manami¹  Yamagata, Tatsuya¹ 

[1] Laboratory of Glycoconjugate Research, Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194, Japan

关键词: Glycosphingolipid;    Glycoside bond;    Solvolysis;    Saccharide analysis;    GLC;    gas liquid chromatography;    HPLC;    high performance liquid chromatography;    Gal-Gb3;    III3 Gal-α-globotriaosylceramide;    Gb4;    globotetraosylceramide;    AsGM2;    asialogangliotriaosylceramide;    HPTLC;    high performance thin layer chromatography;    GLC-MS;    gal liquid chromatography-mass spectrometry;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/0014-5793(91)81262-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rates of acid-catalyzed solvolysis of glycoside bonds in glycosphingolipids were compared to establish a basis for conducting saccharide analysis. Permethylated globotetraosylceramide and asialogangliotriaosylceramide as model compounds for methylation and sugar composition analysis, respectively, were solvolyzed under acidic conditions and the sugar components thus obtained were determined at specified times by gas liquid chromatography, after they had been derivatized. Reducing-end glucose residues in both compounds were liberated more slowly than other sugar residues. Glycoside bonds between reducing-end glucose and ceramide in glycosphingolipids would thus appear to be more resistant towards acid-catalysed solvolysis than other glycoside bonds between hexopyranosides.

【 授权许可】

Unknown