Bond cleavages of adenosine 5'-triphosphate induced by monochromatic soft X-rays

1st Conference on Light and Particle Beams in Materials Science 2013

物理学;材料科学

Fujii, K.^1 ; Narita, A.^1 ; Yokoya, A.^1

Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokyo, Naka, Ibaraki, Japan^1

关键词: Bond cleavages;    Glycoside bond;    Oxygen K-edge;    Phosphate sites;    Soft X-ray beamline;    Spectral change;    X-ray absorption near-edge structure;    XANES spectra;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/502/1/012034/pdf
DOI  :  10.1088/1742-6596/502/1/012034

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

To investigate which type of bond is likely to be cleaved by soft X-ray exposure to an adenosine 5'-triphosphate (ATP), we observed spectral changes in X-ray absorption near edge structure (XANES) around nitrogen and oxygen K-edge of an ATP film by soft X-ray irradiation. Experiments were performed at a synchrotron soft X-ray beamline at SPring-8, Japan. The XANES spectra around the nitrogen and oxygen .K-edge slightly varied by exposure to 560 eV soft X-rays. These changes are originated from the cleavage of C-N bonds between a sugar and a nucleobase site and of C-O, P-O or O-H bond of sugar and phosphate site. From the comparison between the change in XANES intensity of σ* peak at nitrogen and that at oxygen K-edges, it is inferred that the C-O, P-O or O-H bond of sugar and phosphate is much efficiently cleaved than the C-N of N-glycoside bond by the exposure of 560 eV soft X-ray to ATP film.