Binding specificities of the lectins from Helix pomatia, soybean and peanut against different glycosphingolipids in liposome membranes

FEBS Letters

Olofsson, Sigvard²  Månsson, Jan-Eric¹ 

[1] Department of Psychiatry and Neurochemistry, University of Göteborg, St. Jörgen's Hospital, S-422 03 Hisings Backa, Sweden;Department of Virology, Institute of Medical Microbiology, University of Göteborg, Guldhedsgatan 10 B, 413 46 Göteborg, Sweden

关键词: Lectin;    Ganglioside;    Glycosphingolipid;    Affinity chromatography;    Liposome membrane;   

DOI  :  10.1016/0014-5793(83)80506-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding specificities of the lectins from Helix pomatia, soybean and peanut against glycosphingolipids containing β-linked terminal D-galactose and N-acetyl-D-galactosamine and the role of sialic acid as a modulator of the binding specificity were investigated. The test system used consisted of liposomes containing the glycosphingolipids and lectins coupled to gel columns. Of the investigated glycosphingolipids only ganglioside GM2 bound to soybean agglutinin while Helix pomatia agglutinin was found to bind only GA2. Peanut agglutinin showed good affinity both for ganglioside GM1 and its asialoderivative GA1.

【 授权许可】

Unknown