Strain improvement of Aspergillus niger for enhanced lipase production

The Journal of General and Applied Microbiology

John Geraldine Sandana Mala¹  Rengarajulu Puvanakrishnan¹  Numbi R. Kamini¹ 

[1] Department of Biotechnology, Central Leather Research Institute

关键词: Aspergillus niger;    bile salts;    hydrocarbons;    lipase;    mutagenesis;    nitrous acid;    UV;   

DOI  :  10.2323/jgam.47.181

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The enhancement of lipase production from Aspergillus niger was attempted by ultraviolet (UV) and nitrous acid mutagenesis, and the mutants were selected on media containing bile salts. Nitrous acid mutants exhibited increased efficiency for lipase production when compared with UV mutants in submerged fermentation. The hyperproducing UV and nitrous acid mutants were further subjected to a second step of mutagenesis to devise an economical and ecofriendly technique for lipase production by the effective use of hydrocarbons. One percent kerosene was found to be optimal for lipase production, and one of the mutant strains NAII exhibited 2.53 times more increased lipase activity than the parental strain did. This investigation indicates a possible role for the A. niger mutant strains in the biodegradation of oil-polluted environments for the development of ecofriendly technologies.

【 授权许可】

Unknown