Utilization of agroindustrial residues for lipase production by solid-state fermentation

Brazilian Journal of Microbiology

Mônica Caramez Triches Damaso²  Moisés Augusto Passianoto¹  Sidinéa Cordeiro De Freitas²  Denise Maria Guimarães Freire¹  Regina Celi Araujo Lago²  Sonia Couri² 

[1] ,Embrapa Agroindústria de AlimentosRio de Janeiro RJ ,Brasil

关键词: lipase;    Aspergillus niger;    solid state fermentation;    agroindustrial residues;    corn oil by-products;    lipase;    Aspergillus niger;    fermentação em estado sólido;    resíduos agroindustriais;    subprodutos do óleo de milho;   

DOI  :  10.1590/S1517-83822008000400015

来源: SciELO

【 摘 要 】

The aim of this work was to produce lipases by solid-state fermentation (SSF) using, as substrate, agroindustrial residue supplemented with by-products from corn oil refining process or olive oil. For a group of ten fungi strains selected in the first steps, the lipase activity obtained by SSF varied from 7.7 to 58.6 U/g of dry substrate (gds). Among the evaluated strains, the Aspergillus niger mutant 11T53A14 was selected by presenting the best enzymatic production. For the fermentation tests, two substrates were also investigated: wheat bran and corn cob, both supplemented with olive oil. The best results were obtained with wheat bran. Additionally, three industrial by-products from corn oil refining (soapstock, stearin and fatty acids) were evaluated as substitutes to the olive oil in the function of lipases production inducer. Among them, soapstock and stearin were the best inducers, whereas fatty acids presented an inhibitor effect. The highest lipase activities using soapstock, stearin and fatty acids were 62.7 U/gds, 37.7 U/gds and 4.1 U/gds, respectively.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License