Protection against Vibrio alginolyticus in crimson snapper Lutjanus erythropterus immunized with a DNA vaccine containing the ompW gene

Diseases of Aquatic Organisms

Ju-Fen Tang¹  Yu-Cong Huang¹  Bei Wang¹  Yu Ding¹  Ji-Chang Jian¹  Shuang-Hu Cai¹  Yi-Shan Lu¹  Zao-He Wu¹ 

关键词: Vibriosis;    Vaccination;    Recombinant plasmid;    Outer membrane protein;    pcDNA;    Aquaculture;   

DOI  :  10.3354/dao02617

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The outer membrane proteins of Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In the present study, the ompW gene was cloned, expressed and purified. A DNA vaccine was constructed by inserting the ompW gene into a pcDNA plasmid. Crimson snapper Lutjanus erythropterus (Bloch) were injected intramuscularly with the recombinant plasmid pcDNA-ompW. The expression of the DNA vaccine was detected in gill, head kidney, heart, liver, spleen and injection site muscle of crimson snapper by RT-PCR 7 and 28 d post-vaccination. The ELISA results demonstrated that the DNA vaccine produced an observable antibody response in all sera of the vaccinated fish. In addition, crimson snapper immunized with the DNA vaccine showed a relative percentage survival (RPS) of 92.53%, indicating effective protection against V. alginolyticus infection.

【 授权许可】

Unknown