In vivo promoter activity of the synthetic Pribnow box

FEBS Letters

Koroleva, O.N.¹  Drutsa, V.L.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Leninskiye Gory, Moscow 119899, USSR

关键词: Recombinant plasmid;    Gal operon;    Pribnow box;    PCR;    SI-mapping;   

DOI  :  10.1016/0014-5793(91)80118-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA), ??? a containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in a specially constructed plasmid. Using the MacConkey agar indicator technique it has been show that these inserts are able to induced an efficient in vivo expression of the gal operon.

【 授权许可】

Unknown