期刊论文详细信息
FEBS Letters | |
In vivo promoter activity of the synthetic Pribnow box | |
Koroleva, O.N.1  Drutsa, V.L.1  | |
[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Leninskiye Gory, Moscow 119899, USSR | |
关键词: Recombinant plasmid; Gal operon; Pribnow box; PCR; SI-mapping; | |
DOI : 10.1016/0014-5793(91)80118-M | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA), ??? a containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in a specially constructed plasmid. Using the MacConkey agar indicator technique it has been show that these inserts are able to induced an efficient in vivo expression of the gal operon.
【 授权许可】
Unknown
【 预 览 】
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RO201912020294424ZK.pdf | 271KB | download |