FEBS Letters | |
Hybrid 5 S ribosomal RNA encoded by a multicopy plasmid is incorporated into ribosomes of Escherichia coli | |
Szeberényi, József1  Apirion, David1  | |
[1] Department of Microbiology and Immunology, Washington University School of Medicine, Box 8093, St. Louis, MO 63110, USA | |
关键词: Hybrid 5 S rRNA; Fingerpriting; Ribosome; Molecular stalk; RNA processing; Recombinant plasmid; | |
DOI : 10.1016/0014-5793(84)80280-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The recombinant plasmid pJR3Δ contains a tandem pair of promoters from rrnA followed by a hybrid 5 S rRNA gene, derived from the two 5 S rRNA genes of the rrnD transcription unit, and a terminator. Escherichia coli cells transformed with this plasmid produce 2–3-times more 5 S rRNA compared to untransformed cells. The growth of cells containing this plasmid is not affected significantly. Although the sequence and the secondary structure of the plasmid-specific 5 S rRNA differ from those of its counterparts (e.g., from 5 S rRNA species encoded by chromosomal genes), it is processed properly and is incorporated into ribosomes.
【 授权许可】
Unknown
【 预 览 】
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