Hybrid 5 S ribosomal RNA encoded by a multicopy plasmid is incorporated into ribosomes of Escherichia coli

FEBS Letters

Szeberényi, József¹  Apirion, David¹ 

[1] Department of Microbiology and Immunology, Washington University School of Medicine, Box 8093, St. Louis, MO 63110, USA

关键词: Hybrid 5 S rRNA;    Fingerpriting;    Ribosome;    Molecular stalk;    RNA processing;    Recombinant plasmid;   

DOI  :  10.1016/0014-5793(84)80280-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The recombinant plasmid pJR3Δ contains a tandem pair of promoters from rrnA followed by a hybrid 5 S rRNA gene, derived from the two 5 S rRNA genes of the rrnD transcription unit, and a terminator. Escherichia coli cells transformed with this plasmid produce 2–3-times more 5 S rRNA compared to untransformed cells. The growth of cells containing this plasmid is not affected significantly. Although the sequence and the secondary structure of the plasmid-specific 5 S rRNA differ from those of its counterparts (e.g., from 5 S rRNA species encoded by chromosomal genes), it is processed properly and is incorporated into ribosomes.

【 授权许可】

Unknown