How does tmRNA move through the ribosome?

FEBS Letters

Aglyamova, Galina V²  Teraoka, Yoshika¹  Ivanov, Pavel V²  Lim, Valery I³  Bogdanov, Alexey A²  Nierhaus, Knud H¹  Zvereva, Maria I²  Dontsova, Olga A²  Shpanchenko, Olga V² 

[1] Max-Planck-Institute für Molekulare Genetik, AG Ribosomen, Ihnestrasse 73, D-14195 Berlin, Germany;Department of Chemistry, M.V. Lomonosov Moscow State University, 119899 Moscow, Russia;Engelhardt Institute of Molecular Biology, Russian Academy of Science, Vavilov str. 32, 119991 Moscow, Russia

关键词: tmRNA;    Ribosome;    Translation;    Cross-linking;    Site-directed mutagenesis;   

DOI  :  10.1016/S0014-5793(02)02310-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To test the structure of tmRNA in solution, cross-linking experiments were performed which showed two sets of cross-links in two different domains of tmRNA. Site-directed mutagenesis was used to search for tmRNA nucleotide bases that might form a functional analogue of a codon–anticodon duplex to be recognized by the ribosomal A-site. We demonstrate that nucleotide residues U85 and A86 from tmRNA are significant for tmRNA function and propose that they are involved in formation of a tmRNA element playing a central role in A-site recognition. These data are discussed in the frame of a hypothetical model that suggests a general scheme for the interaction of tmRNA with the ribosome and explains how it moves through the ribosome.

【 授权许可】

Unknown