Evidence for a Competitive‐Displacement Model for the initiation of protein synthesis involving the intermolecular hybridization of 5 S rRNA, 18 S rRNA and mRNA

FEBS Letters

Sarge, Kevin D.¹  Maxwell, E.Stuart¹ 

[1] Department of Biochemistry, NCSU Box 7622, North Carolina State University, Raleigh, NC 27695-7622, USA

关键词: Translation;    Ribosome;    Intermolecular RNA:RNA hybridization;    rRNA;    mRNA;   

DOI  :  10.1016/0014-5793(91)81437-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown that a 5′-terminal region of mouse 5 S rRNA can base-pair in vitro with two distinct regions of 18 S rRNA. Further analysis reveals that these 5 S rRNA-complementary sequences in 18 S rRNA also exhibit complementarity to the Kozak consensus sequence surrounding the mRNA translational start site. To test the possibility that these 2 regions in 18 S rRNA may be involved in mRNA binding and translational initiation, we have tested, using an in vitro translation system, the effects of DNA oligonucleotides complementary to these 18 S rRNA sequences on protein synthesis. Results show that an oligonucleotide complementary to one 18 S rRNA region does inhibit translation at the step of initiation. We propose a Competitive-Displacement Model for the initiation of translation involving the intermolecular base-pairing of 5 S rRNA 18 S rRNA and mRNA.

【 授权许可】

Unknown