The Diversity of Vibrios Associated with Vibriosis in Pacific White Shrimp (Litopenaeus vannamei) from Extensive Shrimp Pond in Kendal District, Indonesia

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Sarjito^1 ; Haditomo, Alfabetian Harjuno Condro^1 ; Desrina^1 ; Djunaedi, Ali^2 ; Prayitno, Slamet Budi^1

Aquaculture Department, Faculty of Fisheries and Marine Science Faculty, Diponegoro University, Jl.Prof. H. Soedarto SH, Tembalang, Semarang

50275, Indonesia^1

Department of Marine Science, Faculty of Fisheries and Marine Science Faculty, Diponegoro University, Jl.Prof. H. Soedarto SH, Tembalang, Semarang

50275, Indonesia^2

关键词: 16s r rnas;    Bacterial isolates;    DNA sequence analysis;    Litopenaeus vannamei;    REP-PCR;    V. parahaemolyticus;    Vibriosis;    White shrimps;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012011/pdf
DOI  :  10.1088/1755-1315/116/1/012011

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Vibriosis out breaks frequently occur in extensive shrimps farming. The study were commenced to find out the clinical signs of white shrimp that was infected by the Vibrio and to identify the bacterial associated with vibriosis in the pacific white shrimp, Litopenaeus vannamei. Bacterial isolates were gained from hepatopancreas and telson of moribund shrimps that were collected from extensive shrimp ponds of Kendal District, Indonesia and cultured on Thiosulfate Citrate Bile Salts Sucrose Agar (TCBSA). Isolates were clustered and identified using repetitive sequence-based polymerase chain reaction (rep-PCR). Three representative isolates (SJV 03, SJV 05 and SJV 19) were amplified with PCR using primers for 16S rRNA, and sequence for further identification. The clinical signs of shrimps affected by vibrio were pale hepatopancreas, weak of telson, dark and reddish coloration of smouth, patches of red colour in part of the body on the carapace, periopods, pleuopods, and telson. A total of 19 isolates were obtained and belong to three groups of genus Vibrios. Result of the 16S DNA sequence analysis, the vibrio found in this study related to vibriosis in white shrimps from extensive shrimp ponds of Kendal were closely related to Vibrio harveyi (SJV 03); V. parahaemolyticus (SJV 05) and V. alginolyticus (SJV 19).