Minor effect of depletion of resident macrophages from peritoneal cavity on resistance of common carp Cyprinus carpio to blood flagellates

Diseases of Aquatic Organisms

Nico Van Rooijen¹  Olga L. M. Haenen¹  Adrie Groeneveld¹  Jeroen P. J. Saeij¹  Geert F. Wiegertjes¹ 

关键词: Cyprinus carpio;    Macrophages;    Clodronate;    Trypanoplasma borreli;    Trypanosoma carassii;   

DOI  :  10.3354/dao057067

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Carp Cyprinus carpio macrophages were depleted by intraperitoneal (i.p.) injection of clodronate-liposomes for the in vivo study of the effect of macrophage depletion on the resistance of carp to infection with blood flagellateparasites. Clodronate released inside the cell induces apoptosis of (murine) macrophages. Following i.p. injection of carp with liposomes alone, but not with Trypanoplasma borreli, neutrophilic granulocytes rapidly migrated from the head kidney tothe peritoneal cavity. The majority of liposomes in the peritoneal cavity were not taken up by newly arrived neutrophilic granulocytes, however, but by resident macrophages. After 2 i.p. injections of clodronate-liposomes, the percentage of macrophagespresent in the peritoneal cavity was significantly reduced, as evaluated by flow cytometry. Macrophage-depleted carp that were infected i.p. with T. borreli suffered from high mortality. However, these fish did not show lethal parasitaemia but didshow clear bacteraemia. Macrophage-depleted carp that were infected i.p. with Trypanosoma carassii showed a minor increase in parasitaemia. In addition, macrophage-depleted carp, immune to T. borreli as a result of having survived a priorinfection, remained immune to i.p. reinfection with T. borreli. Succesful depletion of peritoneal macrophages seemed to have a minor effect on the resistance of carp against blood flagellates. However, carp macrophages are essential as a first lineof defence against (bacterial) infection.

【 授权许可】

Unknown