Some immune parameters in carp Cyprinus carpio susceptible and resistant to the haemoflagellate Trypanoplasma borreli

Diseases of Aquatic Organisms

W. Leibold¹  D. Steinhagen¹  J. P. Scharsack¹  W. Körting¹  B. Wagner¹  H. J. Schuberth¹ 

关键词: Trypanoplasma borreli;    Disease susceptibility;    Leukocyte activation;    Immune modulation;    Specific immune responses;   

DOI  :  10.3354/dao060041

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The present study addresses aspects of the (specific) immune response of carp to the haemoflagellate Trypanoplasma borreli. Sera of resistant carp contained antibodies, which agglutinated the flagellates in vitro. When flagellateswere incubated in fish sera from resistant carp, binding of antibodies to flagellates could be demonstrated by flow cytometry, and T. borreli were effectively killed. Heat-treatment of the sera prevented killing, indicating that complementactivation is important for the control of a T. borreli infection. Sera of carp that were highly susceptible to infection with T. borreli contained no antibodies capable of binding to or killing the parasite. Furthermore, specific antibodieswere not generated after experimental infection. This lack of antibody production in susceptible carp is not due to a general unresponsiveness of lymphoid cells, since peripheral blood leukocytes (PBL) from susceptible and resistant carp responded tomitogenic stimuli in vitro with lymphocyte proliferation in a similar manner. However, viable flagellates were significantly less able to stimulate proliferation of PBL from susceptible carp. In vitro-produced culture supernatants of freshlyisolated PBL from both carp lines (but not those of head kidney cells) differentially modulated the mitogen-induced proliferation of PBL from susceptible and resistant carp. The supernatants enhanced the proliferation of leukocytes obtained fromindividuals from the same carp line, but suppressed the mitogen-induced proliferation of PBL from the other line tested. This indicates that lymphoid cells from susceptible and resistant carp differ in their spectrum of spontaneously producedimmunomodulatory mediators. Whether this is decisive for a T. borreli-specific and successful immune response is discussed.

【 授权许可】

Unknown