Density‐dependent induction of TNF‐α release from human monocytes by immobilized P‐selectin

FEBS Letters

Kudo, Sanae²  Irimura, Tatsuro¹  Koike, Junzo¹  Nagata, Kisaburo¹  Tsuji, Tsutomu¹ 

[1] Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;Department of Microbiology, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan

关键词: P-selectin;    Cell adhesion;    Leukocyte activation;    Cytokine production;    Tumor necrosis factor-α;    TNF-α;    tumor necrosis factor-α;    sLeX;    sialyl Lewis X;    FBS;    fetal bovine serum;    PBS;    phosphate-buffered saline;    BSA;    bovine serum albumin;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(00)01765-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

P-selectin purified from human platelets, when immobilized on a solid surface, induced monocytes to release tumor necrosis factor-α (TNF-α). The induction of TNF-α release was dependent on the concentration of P-selectin used for the immobilization, and the maximal stimulation was observed when the plate was coated with 0.3 μg/ml of P-selectin. Use of either a higher or a lower concentration of P-selectin for the plate-coating was found to elicit less TNF-α release, although the higher concentration of P-selectin caused a stronger adhesion of HL-60 leukemic cells. The expression of mRNA for TNF-α roughly paralleled the TNF-α secretion, as assessed by RT-PCR. These results indicate that monocytes are activated by immobilized P-selectin in a density-dependent manner.

【 授权许可】

Unknown