Workers' cytokines profiling upon exposure to MWCNT aerosol in occupational settings

3rd International Youth Conference on Interdisciplinary Problems of Nanotechnology, Biomedicine and Nanotoxicology

物理学;医药卫生;生态环境科学

Fatkhutdinova, L.M.^1 ; Khaliullin, T.O.^1 ; Zalyalov, R.R.^1 ; Vasilyeva, O.L.^1 ; Valeeva, I Kh^2 ; Mustafin, I.G.^3

Department of Hygiene, Occupational Medicine, Kazan State Medical University, Butlerova str., 49, Kazan, Russia^1

Central Laboratory, Kazan State Medical University, Butlerova str., 49, Kazan, Russia^2

Department of Biochemistry, Kazan State Medical University, Butlerova str., 49, Kazan, Russia^3

关键词: Cytokine production;    Epidemiological studies;    Exposure and risk assessments;    Inflammatory response;    Occupational exposure;    Regression model;    Sputum samples;    Systemic response;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/98/1/012031/pdf
DOI  :  10.1088/1757-899X/98/1/012031

来源: IOP

【 摘 要 】

Recent studies have found that upon pulmonary exposure to multi-walled carbon nanotubes (MWCNTs) animals develop primarily fibrosis and granulomas in lungs. In vitro and in vivo studies also give reason to assume that local exposure could be related to remote effects, including immune system and the endothelium. To investigate the remote effect hypothesis, we have analyzed blood, nasal lavage and induced sputum samples taken from workers in the frame of the Russian epidemiological study on Carbon Nanotubes Exposure and Risk Assessment (CNT-ERA). In serum and nasal lavage no significant differences between exposure and control groups were observed with a high variability to the cytokines content. In the samples of induced sputum from exposed workers the content of IL-1b, IL-6, IL-8, TNF-a, IL-4, IL-5, IFN-g exceeded the control group values, but after the regression models construction and bootstrap analysis, significant differences were found only for IL-1b. This study could not provide evidences of blood cytokines changes following local cytokine production in airways in workers exposed to MWCNTs. Cytokines variability in serum and nasal lavage may indicate the absence of severe systemic inflammatory response upon the existing occupational exposure to MWCNTs. Other systemic responses (including allergy-like or autoimmune reactions) should be regarded as well.