Sulfochitosan inhibits P-selectin-mediated HL-60 leukocyte adhesion under flow conditions

Cellular & Molecular Biology Letters

Ximing Liu¹  Ruifei Wang¹  Min Wei¹  Jinfeng Huang¹  Xianlu Zeng¹ 

[1] Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun, P. R. China$$

关键词: Chitosan;    Sulfated chitosan;    P-selectin;    Inhibition;    Cell adhesion;    Flow condition;    Heparin;   

DOI  :  10.2478/s11658-013-0084-1

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Excessive trafficking of leukocytes can lead to serious tissue injury. Here, four regioselectively sulfated chitosans were assessed as inhibitors of HL-60 leukocyte binding to P-selectin, by investigating their effect on leukocyte adhesion to CHO cells expressing human P-selectin under static and flow conditions. The results show that the sulfochitosans exhibit inhibitory activity in this general order: heparin > N-sulfated/6-O-sulfated chitosan ≥ 3-O,6-O-sulfated chitosan > 6-O-sulfated chitosan ≫ N-sulfated chitosan. This suggests that the sulfation of the double site in chitosan is essential for efficient inhibition of P-selectin-mediated HL-60 leukocyte adhesion and that such sulfochitosans may have potential as therapeutic agents against inflammatory disease.

【 授权许可】

Unknown