Effectivity Test Of Crude Protein Spore of Myxobolus koi as Materials Development For Sub Unit Vaccine To Prevent the Gold Fish (Cyprinus carpio, Linn) Dead by Myxobolusis

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Kismiyati^1 ; Mahasri, G.^2

Department of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Kampus C Mulyorejo, 60115, Indonesia^1

Department of Fish Health Management and Aquaculture Faculty of Fisheries and Marine, Universitas Airlangga, Kampus C Mulyorejo, 60115, Indonesia^2

关键词: Antibody production;    Crude proteins;    Cyprinus carpio;    Experimental methods;    Indonesia;    Materials development;    Polyclonal;    Survival rate;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012105/pdf
DOI  :  10.1088/1755-1315/116/1/012105

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Production of fisheries culture according to totality estimated is increasing about 4.9% per year. Gold fish is one of species that have economically value in Indonesia, but there are problem because of myxobolusis. The aims of this research are : Isolation of crude spore protein of Myxobolus koi by using SDS-PAGE and to analyze profile of Myxobolus koi protein that will be developed for sub unit vacsine that can protect koi fish infected by Mxobolus sp. The method of this research used experimental method and belonged to stage : Culture of Myxobolus by using spore scattered, characterization of Myxobolus proteine spore by using SDS-PAGE, Antibody polyclonal production by using ELISA methode to find out optical density of this antibody, and vaccination was done by dipping, with dose 600 μl per liter. The result showed that there were 5 bands of crude protein spore of Myxobolus koi with molecule weight (MW) 41.1 kDa, 51 kDa, 89.8 kDa, 121.7 kDa and 230,1 kDa. The highest Optical Density (0.699) from polychlonal antibody production by using crude spore protein of Myxobolus koi happened on the 42nd day. The result of the chellence test of the protein showed that the highest of leucocyte count happened on the fish immunized by crude spore protein and not infected by Myxobolus koi, but can increase the survival rate (SR) from 20% to 86,37%, so the crude spore protein of Myxobolus can be used for developtment of material for sub unit vaccine to prevent the myxobolusis.