Development of Spore Protein of Myxobolus koi as an Immunostimulant for Prevent of Myxobolusis on Gold Fish (Cyprinus carpio Linn) by Oral Immunisation

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Mahasri, Gunanti^1

Department of Fish Health Management and Aquaculture, Faculty of Fisheries and Marine, Airlangga University, 031-5914151, Indonesia^1

关键词: Crude proteins;    Cyprinus carpio;    Experimental methods;    Indonesia;    SDS PAGE;    Survival rate;    Two bands;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012009/pdf
DOI  :  10.1088/1755-1315/55/1/012009

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Production of Gold fish (Cyprinus carpio Linn) in Indonesia has always increased from 2013 to 2015 year by year with increasing average 2% per year. The amount of production was respectively 571.892 tonnes, 1129.273 tonnes, and 1186.674 tonnes. There were almost no problems to sale of gold fish because it had a good enough prospect. The aims of this research were Isolation of spore protein of Myxobolus koi by using SDS-PAGE to analyze immun respons and survival rate gold fish that immunized with spore protein of Myxobolus koi. The method of this research used experimental method, and belonged to 4 treatments that are: Controle = the group of gold fish not immunized with protein spore of Myxobolus koi neither infected by Myxobolus koi (T1). The group immunized and infested by spore of Myxobolus koi (T2), The group which immunized and not infested by Myxobolus koi (T3), and The group only infested by Myxobolus koi (T4). The dose of immunostimulant was 5 ml in 1 kg of food. The result showed that there were two bands of whole spore protein with molecule weight (MW) 150 kDa and 72 kDa and one band of crude protein Myxobolus koi with molecule weight 73 kD and the optical density point was 0.132 on the first day and increased to 0.769 on the 56thday. The result also showed that the immun respons and survival rate increased from 27% to 86% in chellence test. The protein spore of Myxobolus koi can used to develops material for immunostimulant and to prevent the myxobolusis.