Role of Collagen in Retinoic Acid-induced Differentiation and Down-regulation of TGF-β Receptors in Rat Preosteoblastic RCT-1 Cells

Endocrine Journal

TOMOKO KIKUCHI²  TOSHIRO FUJITA²  YOSHIAKI KODAMA¹  TOSHIO MATSUMOTO²  TAKAHIDE KUROKAWA¹  YASUHIRO TAKEUCHI² 

[1] Department of Orthopedic Surgery University of Tokyo School of Medicine;Fourth Department of Internal Medicine, University of Tokyo School of Medicine

关键词: Osteoblastic differentiation;    Retinoic acid;    Alkaline phosphatase;    Type I collagen;    Transforming growth factor-β (TGF-β) receptors;   

DOI  :  10.1507/endocrj.44.375

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(19)Cited-By(2)Retinoic acid induces differentiation of preosteoblastic cells. We have demonstrated that osteoblastic differentiation and down-regulation of transforming growth factor (TGF)-β receptors requires the interaction of type I collagen with α2β1 integrin (J Biol Chem 271: 3938-3944, 1996). The purpose of this study was to clarify the role of collagen in retinoic acid-induced differentiation and down-regulation of TGF-β receptors using preosteoblastic RCT-1 cells. Retinoic acid enhanced the expression of alkaline phosphatase and type I collagen, and reduced TGF-β receptors in these cells. Inhibiting collagen synthesis abolished these changes. Because TGF-β inhibits osteoblastic differentiation, the changes described here may contribute to the osteoblastic differentiation by retinoic acid.

【 授权许可】

Unknown