Fracture healing induces expression of the proto‐oncogene c‐fos in vivo Possible involvement of the Fos protein in osteoblastic differentiation

FEBS Letters

Konishi, Junji¹  Lee, Kaechoong¹  Yamamuro, Takao²  Ikeda, Toshihiko²  Kasai, Ryuichi²  Ohta, Shuichi²  Hiraki, Yuji³  Kikuchi, Haruki¹  Iwasaki, Rempei²  Shigeno, Chohei¹  Okumura, Hideo² 

[1] Department of Nuclear Medicine and Radiology, Kyoto University Faculty of Medicine, Sakyo, Kyoto 606, Japan;Department of Orthopaedic Surgery, Kyoto University Faculty of Medicine, Sakyo, Kyoto 606, Japan;Department of Biochemistry, Osaka University Faculty of Dentistry, Suita, Osaka 565, Japan

关键词: Fracture healing;    c-fos;    Osteoblastic differentiation;    Alkaline phosphatase;   

DOI  :  10.1016/0014-5793(91)80757-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we report marked in vivo expression of the c-fos gene in the external soft callus (ESC) and periosteal hard callus (PHC) at the fracture site of adult rat tibia. Northern-blot analysis showed that the ESC expressed a high level of c-fos mRNA from post-fracture day 10 to day 28, the time when endochondral ossification progressed, and that the ossifying PHC also expressed c-fos mRNA. This c-fos expression was followed by sequential expression of the genes for alkaline phosphatase, osteopontin and osteocalcin, which are osteoblastic markers. Immunohistochemical analysis showed that the c-Fos protein was predominantly located in osteoblasts in the ossifying calluses.

【 授权许可】

Unknown