Bone morphogenetic proteins (BMP‐2 and BMP‐3) induce the late phase expression of the proto‐oncogene c‐fos in murine osteoblastic MC3T3‐E1 cells

FEBS Letters

Lee, Kaechoong⁴  Yamamuro, Takao³  Kasai, Ryuichi³  Hiraki, Yuji¹  Kohno, Hiroaki³  Shigeno, Chohei⁴  Konishi, Junji⁴  Rosen, David M.²  Ikeda, Toshihiko³  Ohta, Shuichi³  Kikuchi, Haruki⁴  Bentz, Hanne²  Suzuki, Fujio¹ 

[1]Department of Biochemistry, Osaka University Faculty of Dentistry, Yamadaoka, Suita, Osaka 565, Japan

[2]Collagen Corporation, Palo Alto, CA 94303, USA

[3]Department of Orthopaedic Surgery, Kyoto University Faculty of Medicine, Sakyo, Kyoto 606-01, Japan

[4]Calcium Laboratory, Department of Radiology and Nuclear Medicine, Kyoto University Faculty of Medicine, Sakyo, Kyoto 606-01, Japan

关键词: Bone morphogenetic protein;    c-fos;    Osteoblastic differentiation;    MC3T3-El;    Alkaline phosphatase;    Osteocalcin;   

DOI  :  10.1016/0014-5793(92)81505-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we report that bone morphogenetic proteins 2 and 3 (BMP-2 and BMP-3) induced marked expression of c-fos mRNA in a biphasic manner, i.e. the late phase (48 to 60 h) as well as the immediate-early phase (0.5 h), in murine osteoblastic MC3T3-El cells in vitro. The BMP-induced late phase c-fos gene expression was temporally associated with the onset of marked expression of the genes for osteocalcin and alkaline phosphatase, differentiation markers of mature osteoblasts. In contrast, none of TGF-β1, 10% FBS, IGF-I and IGF-II, which induced only the immediate-early c-fos mRNA expression, stimulated the expression of osteocalcin and alkaline phosphatase genes. These data suggest that in osteoblasts BMP-2 and BMP-3 induce the late phase expression of c-fos, which may play a role in transcriptional activation of the genes involved in differentiation of osteoblasts.

【 授权许可】

Unknown