Functional modes of retinoic acid in mouse osteoblastic clone MC3T3‐E1, proved as a target cell for retinoic acid

FEBS Letters

Nakayama, Yasuhiro¹  Nishijima, Katsumi¹  Taniguchi, Shigehiko¹  Nqji, Sumihare¹  Muto, Kohki¹  Takahashi, Kojiro¹ 

[1] Okayama University Dental School, 2-5-1 Shikata-cho, Okayama City 700, Japan

关键词: Retinoic acid;    Retinoic acid receptor gene;    Epidermal growth factor;    Alkaline phosphatase;    Osteoblastic clone;    (Mouse;    Calvaria);   

DOI  :  10.1016/0014-5793(90)80644-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mouse osteoblastic clone MC3T3-E1 was proved as a target cell for retinoic acid (RA) in bone tissues through the demonstration of RA-receptor gene expression by the northern blot analysis. The effect of RA on the cell growth of MC3T3-E1 was repressive for both subconfluent and confluent growth, whereas RA enhancement of alkaline phosphatase expression was observed at the confluent stage. This implies that RA is a regulatory factor leading osteogenesis of the cells after the confluent stage. RA exhibited simultaneously the stage-dependent effects on EGF-dependent mitogenesis: promotive at the subconfluent, but repressive at the confluent stage.

【 授权许可】

Unknown