Retinoic acid controls expression of epidermal transglutaminase at the pre‐translational level

FEBS Letters

Michel, Serge¹  Reichert, Uwe¹  Schmidt, Rainer¹  Isnard, Jean Luc¹  Shroot, Braham¹ 

[1] Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, F-06565 Valbonne, France

关键词: Keratinocyte;    Retinoic acid;    Transglutaminase;    DMEM;    Dulbecco's modification of Eagle's medium containing 5.5 mM glucose and 4 mM glutamine;    DTE;    Dithioerythritol;    EDTA;    Ethylenediamine tetraacetic acid;    EGF;    Epidermal growth factor;    FCS;    Fetal calf serum;    F12;    Ham's medium F12;    NHK;    Normal human keratinocytes;    PBS;    Phosphate-buffered saline;    SDS;    Sodium dodecylsulfate;    TGc;    Cytosoluble transglutaminase;    TGm;    Plasma membrane associated transglutaminase;    Tris;    Tris(hydroxymethyl)aminomethane;   

DOI  :  10.1016/0014-5793(89)81609-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human epidermal keratinocytes were cultured until sub-confluence in low Ca²⁺ (0.15 mM) serum-free synthetic MCDB 153 medium. Raising the Ca²⁺ concentration to 1.15 mM caused an increase in envelope competence as well as plasma membrane associated transglutaminase (TGm) activity. This increase was not observed when the high Ca²⁺ medium contained retinoic acid. Immunofluorescence studies as well as immunoblotting with the TGm-specific monoclonal antibody B.C1 revealed that retinoic acid inhibits expression of TGm. Isolation and in vitro translation of mRNA with subsequent immunoprecipitation showed that retinoic acid inhibits TGm expression at the pretranslational level.

【 授权许可】

Unknown