Plasma membrane transglutaminase and cytosolic transglutaminase form distinct envelope‐like structures in transformed human keratinocytes

FEBS Letters

Michel, Serge¹  Reichert, Uwe¹  Schmidt, Rainer¹  Shroot, Braham¹ 

[1] Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, F-06565 Valbonne, France

关键词: Transglutaminase;    Keratinocyte;    Protein cross-linking;    Pseudo-envelope;    DMEM;    Dulbecco's modification of Eagle's medium containing 5.5 mM glucose and 4 mM glutamine;    DTE;    dithioerythritol;    F12;    Ham's medium F12;    NHK;    normal human keratinocytes;    PBS;    phosphate buffered saline;    TGc;    cytosolic transglutaminase;    TGm;    plasma membrane transglutaminase;    Tris;    tris(hydroxymethyl)aminomethane;   

DOI  :  10.1016/0014-5793(88)80825-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cross-linked envelope formation in the transformed human keratinocyte line SV-K14 requires treatment of the cells with a Ca²⁺ ionophore. Depending on the culture conditions, different extracellular Ca²⁺ concentrations are necessary to trigger the process which is catalyzed by the enzyme transglutaminase. Confluent cells grown in the presence of serum express only the cytosoluble form of the enzyme and need 5 mM Ca²⁺ for optimum protein cross-linking, whereas serum-starved cells which additionally contain the plasma membrane associated form of the enzyme require only 1 mM Ca²⁺. The envelope-like structures thus synthesized are morphologically and biochemically distinct.

【 授权许可】

Unknown