期刊论文详细信息
FEBS Letters
HIV‐1 gp120‐dependent induction of apoptosis in antigen‐specific human T cell clones is characterized by ‘tissue’ transglutaminase expression and prevented by cyclosporin A
Oliverio, Serafina2  Lombardi, Giovanna1  Amendola, Alessandra2  Piacentini, Mauro2  Colizzi, Vittorio2 
[1] Department of Cell Biology and Development, University of Rome ‘La Sapienza’, Rome, Italy;Department of Biology, University of Rome ‘Tor Vergata’, Via della Ricerca Scientifica 1, 00133 Rome, Italy
关键词: AIDS;    Protein cross-linking;    Immune suppresive agent;   
DOI  :  10.1016/0014-5793(94)80427-3
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

We investigated the effect of cyclosporin (CsA) on HIV-gpl20-dependent induction of cell death by apoptosis of human T cell clones specific for influenza virus haemagglutinin and restricted by HLA-DR1. Preincubation of the clones with gp120 induced a large inhibition of their proliferation which was paralleled by the induction of apoptosis. Exposure to the specific antigen alone was able to trigger apoptosis in a significant fraction of cells, this effect was potentiated by pretreatment with gp120. Apoptosis was characterized by the typical morphological changes and by the expression of ‘tissue’ Transglutaminase (tTG), one of the few characterized effector elements of programmed cell death. Interestingly, the tTG protein induction was detectable within the first 24 hours following the gp120 treatment and preceded the appearance of the typical apoptotic phenotype. Noteworthy, CsA treatment prevented the gp120-dependent induction ofapoptosis by blocking the activation of the Ca2+-dependent effector elements such as tTG.

【 授权许可】

Unknown   

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