Mutual arrangement of histone H1 molecules in extended chromatin

FEBS Letters

Nikolaev, Lev G.¹  Glotov, Boris O.¹  Dashkevich, Viktor K.¹  Barbashov, Sergei F.¹  Severin, Eugenii S.¹ 

[1] Institute of Molecular Biology, USSR Academy of Sciences, Moscow 117984, USSR

关键词: Chromatin structure;    Histone H1 arrangement;    Protein cross-linking;    Chymotryptic digestion;    EDTA;    ethylenediamine tetraacetic acid;    disodium salt;    SDS;    sodium dodecyl sulphate;    MMB;    methyl-4-mercaptobutyrimidate-HCl;    TEA;    triethanolamine;   

DOI  :  10.1016/0014-5793(83)81164-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mutual arrangement of histone H1 molecules in chromatin extended in low salt—EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations; i.e., C—C, C—N and N—N. The results imply that the mutual arrangement of H1 histones is determined by the structure of extended nucleosomal chain, rather than chromatin superstructure.

【 授权许可】

Unknown