Retinoic acid modulates retinoid X receptor α and retinoic acid receptor α levels of cultured brown adipocytes

FEBS Letters

Picó, Catalina¹  Ribot, Joan¹  Vázquez, Francisca¹  Bonet, M.Luisa¹  Serra, Francisca¹  Puigserver, Pere¹  Palou, Andreu¹ 

[1] Laboratori de Biologia Molecular, Nutrició i Biotecnologia, Dpt. Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Crta. Valldemossa Km 7.5, Palma de Mallorca 07071, Spain

关键词: Uncoupling protein;    Retinoic acid receptor α;    Retinoid X receptor α;    Retinoic acid;    Cultured brown adipocytes;    BAT;    brown adipose tissue;    UCP;    uncoupling protein;    RA;    retinoic acid;    RAR;    RA receptor;    RXR;    retinoid X receptor;    tRA;    all-trans-RA;    DMEM;    Dulbecco's modified Eagle's medium;    PBS;    phosphate-buffered saline;    PMSF;    phenylmethylsulfonyl fluoride;    ECL;    enhanced chemiluminescence;    CHO cells;    Chinese hamster ovary cells;   

DOI  :  10.1016/S0014-5793(97)00274-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel potential regulatory pathway of brown adipose tissue (BAT) thermogenesis was recently recognized after identifying retinoic acid (RA) as a transcriptional activator of the uncoupling protein (UCP) gene. Here we provide evidence that the UCP responsiveness to RA in primary cultures of brown adipocytes involves RA receptor α (RARα), and show, in the same system and also in CHO cells, that RA down-regulates the steady-state levels of RARα and especially of retinoid X receptor α, suggesting autoregulation of the retinoid pathway and therefore supporting the idea of a physiological role for it in controlling the thermogenic capacity of BAT.

【 授权许可】

Unknown