Modulation of cholinergic locus expression by glucocorticoids and retinoic acid is cell‐type specific

FEBS Letters

Berse, Brygida¹  Blusztajn, Jan Krzysztof¹ 

[1] Department of Pathology, Boston University School of Medicine, Boston, MA 02118, USA

关键词: Choline acetyltransferase;    Vesicular acetylcholine transporter;    Glucocorticoid;    Retinoic acid;    PC12 cell;    SN56 cell;    ACh;    acetylcholine;    ChAT;    choline acetyltransferase;    DMEM;    Dulbecco's modified Eagle medium;    FBS;    fetal bovine serum;    GR;    glucocorticoid receptor;    GRE;    glucocorticoid response element;    GT;    guanidinium isothiocyanate;    RARα;    retinoic acid receptor alpha;    SSC;    saline-sodium citrate;    t-RA;    all-trans-retinoic acid;    VAChT;    vesicular acetylcholine transporter;   

DOI  :  10.1016/S0014-5793(97)00568-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Modulation of mRNA expression of choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) by the glucocorticoid dexamethasone and by retinoic acid was examined in two neuronal cell lines: basal forebrain-derived SN56 and pheochromocytoma PC12. Dexamethasone up-regulated ChAT and VAChT in SN56 cells, while it had inhibitory effects on these genes in PC12 cells. Retinoic acid stimulated the cholinergic markers in both cell types, but in SN56 cells its effect was partially additive with that of dexamethasone, whereas it was much smaller and abrogated by dexamethasone in PC12 cells. Acetylcholine content correlated with these mRNA changes. The presence of a glucocorticoid response element consensus sequence in the VAChT/ChAT gene locus suggests direct transcriptional regulation by glucocorticoids.

【 授权许可】

Unknown