Cholinergic differentiation of cultured sympathetic neurons induced by retinoic acid

FEBS Letters

Matsuoka, Ichiro¹  Kobayashi, Miwako¹  Kurihara, Kenzo¹ 

[1] Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, 060 Japan

关键词: Transmitter phenotype;    Sympathetic neuron;    Choline acetyltransferase;    Tyrosine hydroxylase;    Retinoic acid;    Polymerase chain reaction;    ChAT;    choline acetyltransferase;    TH;    tyrosine hydroxylase;    RA;    retinoic acid;    CNTF;    ciliary neurotrophic factor;    LIF;    leukemia inhibitory factor;    TPA;    phorbol 12;    13-myristate acetate;    PKC;    protein kinase C;    SCG;    superior cervical ganglion;    RT-PCR;    reverse transeription-polymerase chain reaction;   

DOI  :  10.1016/0014-5793(94)80204-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we show that retinoie acid (RA) has the ability to alter the transmitter phenotype of cultured sympathetic neurons from newborn rats superior cervical ganglia (SCG). In the presence of RA, the level of choline acetyltransferase (ChAT) mRNA was increased, while the level of tyrosine hydroxylase (TH) mRNA was reduced in the cultured SCG neurons. Selective PCR amplification of different upstream regions of the ChAT-mRNA indicates that RA promotes the transcription of ChAT gene from R and M exons. The RA-induced upregulation of ChAT-mRNA level was significantly diminished by the chronic treatment with phorbol ester, suggesting that PKC has an important role in the induction of ChAT-mRNA in this system.

【 授权许可】

Unknown