The maspin expression in canine mammary tumors: an immunohistochemical and molecular study

Pesquisa Veterinária Brasileira

Zuccari, Debora A.P.C.²  Gavioli, Arieli F.¹  Bertollo, Eny M. Goloni⁴  Terzian, Ana Carolina B.⁴  Mancini, Ulises M.⁴  Frade, Cibelli S.⁴  Ruiz, Camila M.³  Pavarino-Bertelli, Érika C.⁴  Tajara, Eloisa H.⁴  Castro, Rodrigo⁴  Carmona-Raphe, Juliana⁴  Pivaro, Luana R.⁴ 

[1] Universidade Estadual Paulista, Araçatuba, Brazil;Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, Brazil;Laboratório de Patologia e Biologia Molecular Veterinária, Campinas, Brazil;FAMERP, São José do Rio Preto

关键词: Maspin;    canine mammary tumor;    immunohistochemistry;    molecular biology;    prognosis.;   

DOI  :  10.1590/S0100-736X2009000200015

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

The serpin maspin, a tumor suppressor in breast cancer was described as an inhibitor of cell migration and inducer of cell adhesion between the basement membrane and extracellular matrix resulting in inhibition of tumor metastasis. In contrast, overexpression of maspin is correlated with poor prognosis in other types of cancer. Little is known about expression, regulation and function of maspin in canine mammary tumors. It was demonstrated in this study, a loss of maspin expression in malignant canine mammary cells compared with a pool of normal canine mammary tissue, analyzed by quantitative real-time PCR; weak maspin expression in malignant canine mammary tumors were observed by immunohistochemistry. It was also demonstrated that a correlation with nuclear maspin expression and a good prognosis. It is suggested that maspin could be used as a prognostic marker in canine mammary neoplasia. Index

【 授权许可】

CC BY-NC