NADPH: Protochlorophyllide Oxidoreductase-Structure, Catalytic Function, and Role in Prolamellar Body Formation and Morphogenesis

Timko, Michael P

关键词: Chlamydomonas;    chlorophyll;    gene expression;    molecular biology;    photosynthesis;   

DOI  :  10.2172/1061451
RP-ID  :  DOE-DE-FG02-97ER20279- Final Report
PID  :  OSTI ID: 1061451
Others  :  Other: UVA-GQ10006

学科分类：生物科学（综合）

美国|英语

来源: SciTech Connect

【 摘 要 】

The biosynthesis of chlorophyll is a critical biochemical step in the development of photosynthetic vascular plants and green algae. From photosynthetic bacteria (cyanobacteria) to algae, non-vascular plants, gymnosperms and vascular plants, mechanisms have evolved for protochlorophyllide reduction a key step in chlorophyll synthesis. Protochlorophyllide reduction is carried out by both a light-dependent (POR) and light-independent (LIPOR) mechanisms. NADPH: protochlorophyllide oxidoreductase (EC 1.3.1.33, abbreviated POR) catalyzes the light-dependent reduction of protochlorophyllide (PChlide) to chlorophyllide (Chlide). In contrast, a light-independent protochlorophyllide reductase (LIPOR) involves three plastid gene products (chlL, chlN, and chlB) and several nuclear factors. Our work focused on characterization of both the POR and LIPOR catalyzed processes.