Fetal bovine serum inhibits neomycin-induced apoptosis of hair cell-like HEI-OC-1 cells by maintaining mitochondrial function

American Journal of Translational Research

Ming Guan¹  Song Gao²  Jia Fang³  Qiusheng Huang⁴  Yongming Ma⁵  Yuhua Zhang⁶  Qiaojun Fang⁷  Jie Zhang⁸  Li He⁹  Kaiming Su^1,10 

[1] Department of Otolaryngology, Affiliated Hangzhou First Peoples Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China;Department of Otolaryngology, Affiliated Peoples Hospital of Jiangsu University, Zhenjiang 212002, China;Department of Otolaryngology, Head-Neck Surgery, Shanghai Jiao Tong University Affiliated Sixth Peoples Hospital, Shanghai 200230, China;Department of Otolaryngology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing 210008, China;Department of Otolaryngology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China;Department of Otolaryngology, The Affiliated Hangzhou Hospital of Nanjing Medical University, Hangzhou 310006, China;Department of Otolaryngology, The Affiliated Hospital of Jiangsu University, Zhenjiang 212002, China;Department of Pediatrics, Hangzhou Childrens Hospital, Hangzhou 310000, China;MOE Key Laboratory of Developmental Genes and Human Disease, State Key Laboratory of Bioelectronics, Institute of Life Sciences, Southeast University, Nanjing 210096, China;Research Institution of Otorhinolaryngology, No. 321 Zhongshan Road, Nanjing 210008, China

关键词: Fetal bovine serum;    HEI-OC-1 cells;    neomycin;    apoptosis;    protection;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient in the culture medium of hair cell-like House Ear Institute Organ of Corti 1 (HEI-OC-1) cells, but there have been no reports about the function of FBS in HEI-OC-1 cell apoptosis. In this study, we found that FBS deprivation alone significantly increased HEI-OC-1 cell apoptosis in the absence of neomycin exposure and that the presence of FBS significantly inhibited HEI-OC-1 cell apoptosis after neomycin exposure compared to FBS-deprived cells. Further, we found that the protective effect of FBS was dose dependent and more effective than the growth factors B27, N2, EGF, bFGF, IGF-1, and heparan sulfate. We also found that FBS deprivation significantly disrupted the expression level of mitochondrial proteins, increased pro-apoptotic gene expression, decreased the mitochondrial membrane potential, and increased reactive oxygen species accumulation in HEI-OC-1 cells after neomycin exposure. These findings indicate that FBS is involved in maintaining the level of mitochondrial proteins, maintaining the balance of oxidant gene expression, and preventing the accumulation of ROS, and thus FBS maintains normal mitochondrial function and inhibits apoptosis in HEI-OC-1 cells after neomycin exposure.

【 授权许可】

CC BY-NC