Addition of G418 and other aminoglycoside antibiotics to mammalian cells results in the release of GPI‐anchored proteins

FEBS Letters

Brodbeck, Urs¹  Stadelmann, Barbara¹  Bütikofer, Peter¹  Küng, Matthias¹ 

[1] Institute of Biochemistry and Molecular Biology, University of Bern, Bühlstrasse 28, CH-3012 Bern, Switzerland

关键词: G418 (Geneticin);    Alkaline phosphatase;    Glycosylphosphatidylinositol;    Transfection;    COS-1 cell;    Human erythrocyte;    AChE;    acetylcholinesterase;    AP;    alkaline phosphatase;    FCS;    fetal calf serum;    GPI;    glycosylphosphatidylinositol;    LDH;    lactate dehydrogenase;    MEM;    Eagle's minimal essential medium;    neo;    neomycin;    PI-PLC;    phosphatidylinositol-specific phospholipase C;    VSG;    variant surface glycoprotein;   

DOI  :  10.1016/S0014-5793(97)00452-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Resistance to the neomycin analogue G418 forms the basis of a dominant marker selection system for mammalian cells transfected with the bacterial neomycin gene. We found that COS-1 cells stably transfected with the neomycin resistance gene had a greater than 50% reduction in cell-associated glycosylphosphatidylinositol (GPI)-anchored alkaline phosphatase (AP). A similarly reduced amount of AP was also observed in wild-type COS-1 cells incubated in the presence of G418 or other aminoglycoside antibiotics. The AP was released from cells into the culture supernatant in its GPI-anchored form. Our data suggest that the G418-induced reduction of AP involves a vesiculation process of COS-1 cells.

【 授权许可】

Unknown